Journal
JOURNAL OF SEPARATION SCIENCE
Volume 35, Issue 19, Pages 2599-2607Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201200514
Keywords
ss-Lactams; Milk; Matrix solid phase dispersion; Quick easy cheap effective rugged safe technique; Ultrasound assisted
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Funding
- European Union (European Social Fund-ESF)
- Greek national funds through the Operational Program Education and Lifelong Learning of the National Strategic Reference Framework (NSRF)-Research Funding Program: Heracleitus II
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The application of ultrasound-assisted matrix solid phase dispersive extraction for the confirmatory analysis of 12 beta-lactam antibiotics in milk by high performance liquid chromatography with photodiode array detection has been proposed herein. Four penicillins (cloxacillin, dicloxacillin, oxacillin, and amoxicillin) and eight cephalosporins (cefaclor, cefadroxil, ceftiofur, cefuroxime, cefoperazone, cefazolin, cephalexin, and cefotaxime) are effectively extracted using a mixed sorbent of Quick Easy Cheap Effective Rugged Safe technique and OASIS HLB providing a matrix free from any endogenous interference. Examined analytes were well resolved on an Inertsil ODS-3 analytical column with a mobile phase of CH3COONH4 (0.05 M) and acetonitrile delivered under a gradient program. 1,7-Dimethyl-xanthine was used as internal standard. The method was validated meeting the European Legislation determining linearity, selectivity, stability, decision limit, detection capability, accuracy, precision, and ruggedness according to the Youden approach. Recoveries of all antibiotics rated from 85.0 to 115.7%, while RSD values were <12.7%. Finally, the method was successfully applied to milk samples purchased from local market.
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