Journal
JOURNAL OF SEPARATION SCIENCE
Volume 35, Issue 20, Pages 2710-2717Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201200344
Keywords
Aminoglycosides; Food of animal origin; Hydrophilic interaction chromatography; Tandem mass spectrometry; Weak cation exchange SPE
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Funding
- Spanish Ministerio de Ciencia e Innovacion [AGL 2008-05578-C05-03]
- Generalitat de Catalunya [2011 FI-B1 00141]
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Two methods based on liquid chromatographytandem mass spectrometry were developed for the determination of ten aminoglycosides (streptomycin, dihydrostreptomycin, spectinomycin, apramycin, paromomycin, kanamycin A, gentamycin C1, gentamycin C2/C2a, gentamycin C1a, and neomycin B) in kidney samples from food-producing animals and in honey samples. The methods involved extraction with an aqueous solution (for the kidney samples) or sample dissolution in water (for the honey samples), solid-phase extraction with a weak cation exchange cartridge and injection of the eluate into a liquid chromatographytandem mass spectrometry system. A zwitterionic hydrophilic interaction chromatography column was used for separation of aminoglycosides and a triple quadrupole mass analyzer was used for detection. The methods were validated according to Decision 2002/657/EC. The limits of quantitation ranged from 2 to 125 mu g/kg in honey and 25 to 264 mu g/kg in the kidney samples. Interday precision (RSD%) ranged from 6 to 26% in honey and 2 to 21% in kidney. Trueness, expressed as the percentage of error, ranged from 7 to 20% in honey and 1 to 11% in kidney.
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