Journal
JOURNAL OF SEPARATION SCIENCE
Volume 35, Issue 12, Pages 1506-1513Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201101067
Keywords
Human serum; Magnetic mesoporous silica spheres; Peptides and phosphopeptides; Titanium
Categories
Funding
- National Nature Science Fund [91017013, 31070327]
- Science Fund for Creative Research Groups [20921062]
- NSFC
- National 973 project of China [2007CB914200]
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Protein phosphorylation is a common posttranslational modification, and involved in many cellular processes. Like endogenous peptides, endogenous phosphopeptides contain many biomarkers of preclinical screening and disease diagnosis. In this work, titanium-containing magnetic mesoporous silica spheres were synthesized and applied for effective enrichment of peptides from both tryptic digests of standard proteins and human serum. Besides, the enriched peptides can be further separated into nonphosphopeptides and phosphopeptides by a simple elution. First, titanium-containing magnetic mesoporous silica spheres were synthesized by a sol-gel method and found to have high surface area, narrow pore size distribution, and useful magnetic responsivity. Then, as the prepared material was used for selective capturing of phosphopeptides, it demonstrated to have higher selectivity than commercial titanium dioxide. Moreover, via combination of size-exclusion mechanism, hydrophobic interaction, and affinity chromatography, titanium-containing magnetic mesoporous silica spheres were successfully applied to simultaneously extract and separate nonphosphopeptides and phosphopeptides from standard protein digestion and human serum.
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