Journal
JOURNAL OF SEPARATION SCIENCE
Volume 35, Issue 8, Pages 915-921Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201101032
Keywords
Biogenic amines; Chemically bonded phases; Computed logP; Lipophilicity; TLC
Categories
Funding
- Sectorial Operational Programme for Human Resources Development
- European Social Fund [POSDRU 89/1.5/S/60189]
- [PN-II-ID-PCE-2011-3-0366]
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The retention behavior for a series of biogenic amines and related sympathomimetic drugs has been investigated in reversed-phase thin-layer chromatography using RP-2, RP-8, RP-18W, and Diol stationary phase and mixtures of phosphate buffer (pH = 7.10) and methanol in different proportions as mobile phases. Several methodologies like arithmetic mean of experimental retention values, extrapolation to zero methanol concentration procedure and principal component analysis were applied to retention data values (RM) in order to determine relevant parameters (mean of RM - mRM, RM0, and scores corresponding to the first principal component - PC1/RM respectively) encoding information on the lipophilic behavior of compounds. High similarities in lipophilicity behavior of investigated amines were highlighted by mRM and PC1/RM lipophilicity indices for all of the studied stationary phases. The experimental results were compared with some computed lipophilicity parameters expressed as distribution coefficients at working pH (logD), partition coefficients (logPN, logPI, and diff(logPNI)) concerning both neutral and fully protonated species and difference between both species, and also with various lipophilicity values (logP) generated by different commonly used software. Significant correlations were observed between the experimental lipophilicity indices mRM respectively PC1/RM and diff(logPNI) values in all cases.
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