Journal
JOURNAL OF SEPARATION SCIENCE
Volume 35, Issue 17, Pages 2210-2218Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201200236
Keywords
Anemarrhena asphodeloides; Comprehensive LC; Polyamine column; Steroid glycoside
Categories
Funding
- National Natural Science Fund of P.R. China [81072614]
- Scientific Foundation of Shanghai China [09DZ1975100]
- Shanghai Key Laboratory for Pharmaceutical Metabolites Research [08DZ2270900]
Ask authors/readers for more resources
In the work presented here, a novel approach to comprehensive two-dimensional liquid chromatography is evaluated. Reversed-phase liquid chromatography was employed for the first-dimension separation and polyamine chromatography was chosen for the second-dimension separation mode. The two dimensions are highly orthogonal and the separation efficacy of the developed octadecylsilica x polyamine was tested by separating an extract from Anemarrhena asphodeloides. The steroid glycosides identified by comprehensive two-dimensional liquid chromatography in this experiment were compared to those obtained for monodimensional liquid chromatography. The comprehensive two-dimensional liquid chromatography system, thanks to the complementary separation selectivity and enhanced peak capacity provided by the two columns, allowed to distribute five compounds of low amounts otherwise unachievable by monodimensional liquid chromatography. In addition, four steroid isomers with similar fragmentation characteristics in MS/MS spectra, were newly separated based on their different chemical structures.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available