Journal
JOURNAL OF SEPARATION SCIENCE
Volume 34, Issue 10, Pages 1180-1183Publisher
WILEY-BLACKWELL
DOI: 10.1002/jssc.201100007
Keywords
Continuous-flow separation; Debye layer overlap; Electrostatic sieving; Nanoparticles; Proteins
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Funding
- German Science Foundation (DFG) within the Collaborative Research Center [SFB 613]
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Continuous-flow separation of nanoparticles (NPs) (15 and 39 nm) is demonstrated based on electrostatic sieving at a micro-nanofluidic interface. The interface is realized in a poly(dimethylsiloxane) device with a nanoslit of 525 nm laterally spanning the microfluidic channel (aspect ratio of 540:1). Within this nanoslit, the Debye layers overlap and generate an electrostatic sieve. This was exploited to selectively deflect and sort NPs with a sorting purity of up to 97%. Because of the continuous-flow operation, the sample is continuously fed into the device, immediately separated, and the parameters can be adapted in real time. For bioanalytical purposes, we also demonstrate the deflection of proteins (longest axis 6.8 nm). The continuous operation mode and the general applicability of this separation concept make this method a valuable addition to the current Lab-on-a-Chip devices for continuous sorting of NPs and macromolecules.
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