Journal
JOURNAL OF SEPARATION SCIENCE
Volume 34, Issue 20, Pages 2893-2900Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201100551
Keywords
CE; Non-covalent labeling; Plant peptide hormone; Quantum dots; Systemin
Categories
Funding
- National Natural Science Foundation of China [90717002, 20905005, 20805001]
- Science Foundation of China [20090450231, 201003008]
Ask authors/readers for more resources
Quantum dots (QDs), with their superior size-dependent fluorescence properties, have been employed as non-covalent fluorescent labels for the determination of tomato systemin (TomSys) by capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection. The optimum conditions of in-capillary labeling and CE separation were investigated in detail, and complete separation of QDs-labeled TomSys from free QDs labels was achieved. Satisfactory results were obtained in terms of linearity (R-2 = 0.998), sensitivity (limit of detection, 66 fmol) and repeatability (run-to-run RSDs of migration time and peak area, 0.9 and 4.6%, respectively; day-to-day RSDs of migration time and peak area, 3.1 and 11.9%, respectively). The established CE-LIF method was later applied in the detection of TomSys spiked in the sample of tomato leaves, which showed the applicability of the proposed method in the analysis of the target plant peptide hormone in the complex matrix.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available