Journal
JOURNAL OF SEPARATION SCIENCE
Volume 34, Issue 19, Pages 2611-2617Publisher
WILEY-BLACKWELL
DOI: 10.1002/jssc.201100205
Keywords
Anthocyanins; Counter-current chromatography; Peptides; Proteins; Spiral tube column
Categories
Funding
- National Natural Science Foundation of China [20877005]
- National High Technology Research and Development Program (863 Program) [2007AA061601]
- Beijing Natural Science Foundation [2102016]
Ask authors/readers for more resources
An improved type-J counter-current chromatography (CCC) planet centrifuge with two spiral tube columns (volume 2 x 15 mL, beta value 0.3-0.7, tubing 0.8 mm id) was developed and evaluated for its retention ability of four typical different solvent systems including heptane-methanol (1:1, v/v) (A), hexane-ethyl acetate-methanol-water (1:1:1:1, v/v) (B), n-butanol-acetic acid-water (4:1:5, v/v) (C), PEG1000-K(2)HPO(4)-water (12.5:12.5:75, w/w) (D) under eight different operation modes. The results indicated that the spiral tube column could significantly increase the retention of four typical solvent systems compared with a traditional multilayer coil column with similar parameters (volume 35 mL, beta value 0.3-0.7, tubing 0.8 mm id). The retention of stationary phase (S(f)) for the less polar system (A) and moderately polar solvent system (B) can be increased by about 10%, and for the polar system (C) and aqueous two-phase system (ATPS) (D) by 30-40%. The preliminary applications of this spiral tube column to the separation of small molecular compounds such as moderately polar theaflavins, polar anthocyanins and dipeptides were successful. Acceptable resolution can be obtained between cytochrome c and myoglobin, lysozyme and myoglobin when it was applied on protein separation; however, it still needs to be improved with regard to its column efficiency.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available