Journal
JOURNAL OF SEPARATION SCIENCE
Volume 33, Issue 15, Pages 2250-2257Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201000197
Keywords
Carotenoids; Cytotoxic; HPLC-PAD-atmospheric pressure chemical ionization-MS; Marthasterias glacialis
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Funding
- FCT [SFRH/BD/62663/2009]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/62663/2009] Funding Source: FCT
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An HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling analysis was conducted on the marine echinoderm Marthasterias glacialis (spiny sea-star). Bio-guided purification of the methanolic extract led to the isolation of several carotenoids, namely zeaxanthin, astaxanthin and lutein. These compounds were characterized using both UV Vis characteristics and MS spectra interpretation. No previous works addressed the MS analysis of carotenoids present in this organism. The purified carotenoid fraction displayed a strong cell proliferation inhibition against rat basophilic leukemia RBL-2H3 (IC25 = 268 mu g/mL) cancer cell line. Against healthy V79 (rat lung fibroblasts (IC25 = 411 mu g/mL)) cell line, however, toxicity was lower, as it is desired for anti-cancer molecules. This study suggests that M. glacialis may constitute a good source of bioactive compounds that can be used as lead compounds for the pharmaceutical industry.
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