Journal
JOURNAL OF SEPARATION SCIENCE
Volume 33, Issue 17-18, Pages 2599-2609Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201000294
Keywords
Cefepime; Goat milk; Goat plasma; HPLC; Pharmacokinetics
Categories
Ask authors/readers for more resources
A highly sensitive and selective H PLC method with UV detection was developed for the determination of cefepime in goat plasma and milk. The proposed method was based on the complexation of cefepime with Hg(I) ions that imparts the high selectivity of the proposed method with enhancement of the sensitivity which enabled the analysis of cefepime in complex matrices such as plasma and milk. Detection was performed at 263 nm, using cefuroxime sodium as an internal standard. Chromatographic separation of cefepime and the internal standard was achieved with Aqua RP-C-18 column using methanol/triethylamine-acetate buffer, pH 3.5 (18:82, v/v) as mobile phase at a flow rate of 1 mL/min. Linear detector responses were observed spanning the range of 1.3-20 mu g/mL. The LOD for standard cefepime was 0.43 mu g/mL, whereas the LOD for cefepime in goat plasma was 0.84 mu g/mL and the corresponding value in goat milk was 1.1 mu g/mL. No interference from endogenous substances in plasma and milk was observed. The developed HPLC method has been successfully applied for the pharmacokinetic study of cefepime in goat plasma and milk, for the first time, after a single intramuscular injection of 50 mg cefepime/kg body weight.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available