Journal
JOURNAL OF SEPARATION SCIENCE
Volume 33, Issue 4-5, Pages 651-657Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200900603
Keywords
Auxin; Cytokinin; Plant hormone; Pressurized CEC; UV detection
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Funding
- National Nature Sciences Foundation of China [20675016, 20735002]
- Ministry of Education of China [708056]
- Key Program of the International Corporation of Science and Technology Department of Fujian Province, China [200710020, 2007Y0060]
- Plan of University New Century Outstanding Talent of Fujian Province [HX2006-101]
- Key Special Purpose Funding of Physical Education Bureau of Fujian Province, China [HX2005-74]
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Pressurized CEC (pCEC) with UV detection was developed for the simultaneous analysis of endogenetic and ectogenic plant hormones. Effects of some crucial factors including mobile phase, buffer concentration, pH value and applied voltage on pCEC separation were investigated, respectively. Under the optimum conditions, namely a packed capillary column (3 mu m Hypersil C-18, 45 cm total length with 100 mu m id x 20 cm effective length) as the separation channel; ACN: 10 mmol/L phosphate (50:50, v/v, %) as the mobile phase, pH 4.0; pump flow rate 0.035 mL/min; separation voltage -10 kV; UV wavelength 222 nm, a complete separation of five hormones was accomplished. The pCEC assay presented good linearity (0.8-100 mu g/mL), with LOD of 0.2 mu g/mL and R in range of 0.998-0.999. Meanwhile, a lack-of-fit test was performed to evaluate these linear models. Results showed that no significant lack-of-fit was observed and these models were appropriate (alpha = 0.05).
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