Journal
JOURNAL OF SEPARATION SCIENCE
Volume 32, Issue 15-16, Pages 2530-2543Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200900202
Keywords
HILIC; Monolithic columns; Phenolic acids; Reversed phase LC; Sulfobetaine stationary phase
Categories
Funding
- Ministry of Education, Youth and Sports of the Czech Republic [MSM 0021627502]
- Grant Agency of Czech Republic [203/07/0641]
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Monolithic columns for capillary hydrophilic interaction liquid chromatography (HILIC) were prepared in fused-silica capillaries by radical co-polymerization of [2(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide and ethylene dimethacrylate in various binary and ternary porogen solvent mixtures with azobisisobutyronitrile as the initiator of the polymerization reaction. Columns showed mixed separation modes: reversed-phase (RP) in water-rich mobile phases and HILIC at high concentrations of acetonitrile (>60-80%) in aqueous-organic mobile phases. A continuous change in retention was observed at increasing concentration of water in acetonitrile, giving rise to characteristic U-turn plots of retention factors versus the concentration of water in the mobile phase, with minima corresponding to the transition between the mechanisms controlling the retention. The selectivity of organic polymer monolithic columns for HILIC separations can be varied by adjusting the concentration of sulfobetaine monomer and the composition of the porogen solvent in the polymerization mixture. Under HILIC conditions, the monolithic capillary sulfobetaine columns show separation selectivities for polar phenolic acids similar to those of a commercial silica-based sulfobetaine ZIC-HILIC column, which, however, has limited selectivity in the RP mode due to lower retention.
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