Journal
JOURNAL OF SEPARATION SCIENCE
Volume 32, Issue 8, Pages 1137-1144Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200800578
Keywords
Comprehensive LC x LC; pH; RP; Serum; Tryptic digests
Categories
Funding
- Science and Technology in Flanders (IWT-Vlaanderen)
Ask authors/readers for more resources
As all alternative to the classical approach of combining strong cation exchange SCX-LC and RP-LC for the separation of complex proteomic samples, this essay describes the online comprehensive RP-LC x RP-LC separation of BSA and human blood serum. High orthogonality and peak capacity are achieved through the application of a significantly different pH in the two dimensions. The coupling of fused-core columns in series ensures high efficiency in the first dimension, while a previously designed interface with parallel second dimension columns further enhances the separation capability of the comprehensive system.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available