Journal
JOURNAL OF SEPARATION SCIENCE
Volume 32, Issue 8, Pages 1200-1210Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200800464
Keywords
Addiction; Astrocytes; Cell Culture; Morphine; Neurons; Proteome
Categories
Ask authors/readers for more resources
Using primary neuronal cell culture assays, combined with 2-D gel electrophoresis and capillary LC-MS, we identified differences in proteomes between control and morphine-treated cells. Statistically significant differences were observed among 26 proteins. Nineteen of them were up-regulated, while seven were down-regulated in morphine-treated cell populations. The identified proteins belong to classes involved in energy metabolism, associated with oxidative stress, linked with protein biosynthesis, cytoskeletal ones, and chaperones. The detected proteins demand further detailed studies of their biological roles in morphine addiction. It is crucial to confirm observed processes in vivo in order to reveal the nature and importance of the biological effect of proteome changes after morphine administration. Further investigations may lead to the discovery of new proteome-based effects of morphine on living organisms.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available