Journal
JOURNAL OF SEPARATION SCIENCE
Volume 32, Issue 11, Pages 1981-1986Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200800562
Keywords
Bovine serum albumin (BSA); Chitosan; High performance liquid chromatography (HPLC); Molecularly imprinted polymers (MIPs)
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Funding
- National Basic Research Program of China (973 program) [2007CB914101]
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A novel approach to the manufacturing of protein-responsive imprints on a home-made chitosan substrate was established together with m-aminophenylboronic acid (APBA) as a functional monomer. The produced polymers were characterized using both (1) equilibrium adsorption assays and (2) high performance liquid chromatography analysis. Results confirmed that the synthesized BSA-MIP (molecularly imprinted polymer) has a high affinity towards its template compared to the determined control proteins. The produced BSA-MIP featured largely in its good adsorption reversibility, especially in competitive binding assays, which is of great biological significance in separations. Non-specific binding was reduced to almost zero in a BSA/BHb competitive binding event. An excellent HPLC profile of template recognition was found for BSA-MIP, even under harsh mobile phase conditions. In the present work, the adopted trapped-template-release method permits recovery of bound BSA [1]. The strategy of making an artificial protein-receptor with high adsorption affinity and reversibility is promising in on-line isolation of target protein from complicated biological environments.
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