Journal
JOURNAL OF SEPARATION SCIENCE
Volume 32, Issue 12, Pages 2066-2074Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200900106
Keywords
Computation methods; Flavonoids; Lipophilicity; Principal component analyis; RP-HPLC
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Funding
- Ministry of Education and Research of Romania [IDEI 560/2007-2010]
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The chromatographic behavior of some flavonoids was established by RP-HPLC on RP-18 (LiChroCART, LiChrosphere RP-18e), RP-8 (Zorbax, Eclipse XDB-C8), CN (Saulen-technik, LiChrosphere CN100) columns. The mobile phases were mixtures of methanol-water in different volume proportions from 70 to 80% v/v for RP-18 and RP-8, while for the CN column the proportions were between 66 and 70% v/v. The lipophilicity was expressed through different lipophilicity descriptors such as mean of k (mk), mean of log k (mlog k), log k(w), S, f(0), and scores of k and log k corresponding to the first principal component. The experimental lipophilicity indices are directly correlated with the computed values, via computer software and internet module, at a high analytical level. Furthermore, the results obtained applying principal component analysis to k and/or log k values allow the prediction and explanation of the interaction involved in the retention mechanism which takes place between the compounds and the employed stationary phases during the development.
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