Journal
JOURNAL OF SEPARATION SCIENCE
Volume 31, Issue 15, Pages 2754-2773Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200800243
Keywords
HILIC/CEX; mixed-mode; peptides proteins; RPC
Categories
Funding
- NIH [R01 GM61855]
Ask authors/readers for more resources
This review represents a summary of the development and application of a novel mixed-mode HPLC approach to the separation and analysis of peptides and proteins termed hydrophilic interaction/cation-exchange chromatography (HILIC/CEX). This approach combines the most advantageous aspects of two widely different separation mechanisms, i.e. a separation based on hydrophilicity/hydrophobicity differences between polypeptides overlaid on a separation based on net charge. Applications described include HILIC/CEX separations of cyclic peptides, a-helical peptides, random coil peptides and modified or deletion products of synthetic peptides. In addition, the excellent resolving ability of HILIC/CEX for modified histone proteins is described. This approach is shown to represent an excellent complement to RP chromatography (RPC), as well as being a potent analytical tool in its own right.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available