Journal
JOURNAL OF SEPARATION SCIENCE
Volume 31, Issue 14, Pages 2598-2604Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200800205
Keywords
inorganic ions; ion-exchange chromatography; matrix elimination; polymer monolith
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Funding
- Australian Research Council's Discovery Projects [DP0450145]
- ARC Federation Fellowship [FF0668673]
- ARC APD Fellowship [DP0453052]
- Australian Research Council [DP0450145, DP0453052] Funding Source: Australian Research Council
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A commercially available 4.6 mm id x 50 mm polymethacrylate-based monolithic strong anion exchange column (ProSwift (TM), SAX-1S) designed for the separation of proteins has been successfully used to separate small inorganic anions in the presence of a seawater sample matrix. Using a hydroxide eluent with suppressed conductivity detection the ion exchange capacity of this column declined over time; however, using KCl as the eluent, the column performance was stable with a capacity of 530 mu equiv. for nitrate. The optimum conditions for the separation of iodate, bromate, nitrite, bromide and nitrate were assessed by constructing van Deemter plots using 1.00 and 0.100 M KCl. Efficiencies of tip to 26 700 plates/m were recorded using 1.00 M KCl, at a flow rate of 0.20 mL/min but iodate was not baseline resolved from the void peak. By reducing the concentration of the eluent to 0.100 M, efficiencies of up to 39 900 plates/m could be obtained at 0.35 mL/min. By employing a linear gradient ranging from 0.05 to 1.00 M KCl the ions dissolved in distilled water or a salt water matrix could be baseline separated in less than 3 min at a flow rate of 2.50 mL/min.
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