Journal
JOURNAL OF SEPARATION SCIENCE
Volume 31, Issue 4, Pages 683-688Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200700409
Keywords
counter-current chromatography; flavonoid glycosides; polyamide resin; purification; taraxacum mongolicum
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A preparative high-speed counter-current chromatography (HSCCC) was successively applied to purify three flavonoid glycosides from the aerial part of Taraxacum Mongolicum, a traditional Chinese medicine. Subsequent UV, MS, and NMR analyses have led to the characterization of three flavonoid glycosides including two new compounds isoetin-7-O-beta-D-glucopyranosyl-2'-O-alpha-L-arabinopyranoside and isoetin-7-O-beta-Dglucopyranosyl-2'-O-alpha-D-glucopyranoside, and a known compound, isoetin-7-O-beta-D-ghtcopyranosyl-2'-O-alpha-D-xyloypyranoside, which were first isolated from T. mongolicum. The two-phase solvent system composed of ethyl acetate/n-butanol/water (2:1:3, v/v/v) was performed in HSCCC. Consequently, a total of 25.7 mg isoetin-7-O-beta-D-glucopyranosyl-2'-O-alpha-L-arabinopyranoside, 19.1 mg isoetin-7-O-beta-D-glucopyranosyl-2'-O-alpha-D-glucopyranoside, and 10.6 mg isoetin-7-O-beta-D-glucopyranosyl-2'-O-alpha-D-xyloypyranoside were obtained with purity of 98.7, 98.3, and 99.1%, respectively, as determined by HPLC from 500 mg enriched extract after cleaning-up by polyamide resin.
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