Journal
JOURNAL OF SEPARATION SCIENCE
Volume 31, Issue 11, Pages 2032-2039Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200800087
Keywords
2-DE; plant proteomics; protein extraction; recalcitrant tissues; sample preparation
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Plant tissues usually contain high levels of proteases and secondary metabolites that severely interfere with protein extraction, separation, and identification. Preparation of high-quality protein samples from plant tissues for proteomic analysis represents a great challenge. This article briefly describes the critical points in protein separation, especially secondary metabolites in plant tissues, and removal strategy. It provides an updated overview of three total protein extraction methods and their applications in proteomic analysis of various recalcitrant tissues.
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