Journal
PLACENTA
Volume 36, Issue 8, Pages 863-869Publisher
W B SAUNDERS CO LTD
DOI: 10.1016/j.placenta.2015.06.010
Keywords
Selenium; Reactive oxygen species; Mitochondrial biogenesis; Antioxidant; Trophoblast
Funding
- Griffith University School of Medical Science Postgraduate Research Account
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Introduction: Placental oxidative stress has been implicated in pregnancy complications and previous work has shown that selenium can protect trophoblast mitochondria from oxidative stress. This report examines mitochondrial function and content in trophoblasts supplemented with selenium. Methods: Swan-71, JEG-3 and BeWo cells and placental tissue were incubated with sodium selenite or selenomethionine. Mitochondrial function was examined in a respirometer. Mitochondrial content was determined using RT-PCR. The levels of the mitochondrial biogenesis markers selenoprotein H, PGC-l alpha and NRF-1 was examined by western blotting. Results: Mitochondrial respiration was significantly enhanced post selenium supplementation in cells and tissues. Selenium supplementation increased mitochondrial content and up-regulated mitochondrial biogenesis mediators in cells. Discussion: These results emphasise the importance of selenium in mitochondrial regeneration in trophoblasts. (C) 2015 Elsevier Ltd. All rights reserved.
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