Kiss1 mutant placentas show normal structure and function in the mouse

Introduction: Kisspeptins, encoded by the Kiss1 gene, are a set of related neuropeptides that are required for activation of the mammalian reproductive axis at puberty and to maintain fertility. In addition, kisspeptin signaling via the G-protein coupled receptor GPR54 (KISS1R) has been suggested to regulate human placental formation and correlations have been found between altered kisspeptin levels in the maternal blood and the development of pre-eclampsia. Methods: We have used Kiss1 and Gpr54 mutant mice to investigate the role of kisspeptin signaling in the structure and function of the mouse placenta. Results: Expression of Kiss1 and Gpr54was confirmed in themouseplacenta but no differences in birthweight were found in mice that had been supported by a mutant placenta during fetal development. Stereological measurements found no differences between Kiss1 mutant andwild-type placentas. Measurement of aminoacid andglucose transport across the Kiss1mutant placentas at E15.5 days did not reveal any functionaldefects. Discussion: These data indicate that mouse placentas can develop a normal structure and function without kisspeptin signaling and can support normal fetal development and growth. (c) 2014 The Authors. Published by Elsevier Ltd.