Journal
JOURNAL OF REPRODUCTION AND DEVELOPMENT
Volume 56, Issue 6, Pages 583-592Publisher
SOCIETY REPRODUCTION & DEVELOPMENT-SRD
DOI: 10.1262/jrd.10-007A
Keywords
Bioanalyzer; Cumulus; Oocyte; RNA; Sperm
Funding
- USDA Cooperative State Research, Education and Extension Service [2004 35203-14772]
- USDA
- state of Tennessee through the Tennessee Agricultural Experiment Station
- Department of Animal Science
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Results described herein provide insight regarding certain features of gamete RNA and how they compare to cumulus cell RNA In particular, 28S/18S rRNA ratio and size distribution of RNA molecules differed in total RNA from oocytes versus surrounding cumulus cells Specifically, oocyte total RNA had a lower rRNA ratio and an increased abundance of smaller RNA sizes compared to RNA from surrounding cumulus Extensive efforts demonstrated that observed differences were repeatable whether oocyte maturation occurred in vitro or in vivo, and were similar between the nuclear stages examined Features of oocyte RNA were conserved across six mammalian species, yet differed from surrounding cumulus Profiles of sperm RNA were also examined but had no discernible ribosomal RNA peaks and were conserved across four mammalian species Because the oocyte and spermatozoon are highly specialized cells representing unique molecular entities required for proper embryo development, dissimilarities described herein likely represent real gamete versus cumulus RNA differences
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