Journal
PHYTOCHEMISTRY LETTERS
Volume 14, Issue -, Pages 153-158Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.phytol.2015.10.003
Keywords
15-Lipoxygenase; Nitric oxide; Natural compounds; RAW264.7 macrophages
Categories
Funding
- University of Pretoria
- National Research Foundation [Eloff 95991]
- Medical Research Council (SIR Eloff)
- Alexander von Humboldt-Stiftung [1137675]
- German Academic Exchange Service (DAAD) [Referat 412, PKZ A/12/904859]
Ask authors/readers for more resources
Species of the genus Garcinia have been the source of many benzophenone and xanthone derivatives. Recent data regarding potent biological properties of natural compounds in Garcinia species led us to investigate the in vitro anti-inflammatory effect of three known xanthones, lichexanthone (1), 1,3,6,7-tetrahydroxyxanthone (2), 1,3,5,6-tetrahydroxyxanthone (3), two new xanthones 1-hydroxy-3,6,7-tri-O, O, O-triprenylxanthone (4), 1,6-dihydroxy-3,7-di-O, O-diprenylxanthone (5) and two benzophenones isoxanthochymol (6), guttiferone E (7), isolated from Garcinia nobilis and Garcinia punctata. The Griess assay was used for the measurement of nitric oxide (NO) production in RAW264.7 macrophages and the ferrous oxidation-xylenol orange assay was used to determine the 15-lipoxygenase (15-LOX) inhibitory activity. All the compounds had inhibitory effect on 15-LOX activity to different extents. Compound (7) had the highest anti-LOX activity with an IC50 value of 43.05 mu g/mL. At the highest studied concentration (25 mg/mL), compound (4) had the most potent inhibitory activity against NO release with a% of inhibition of 95.42% and less cytotoxic effect on RAW264.7 cells (% of cell viability of 81.40). The results presented here suggest that 1,3,5,6-tetrahydroxyxanthone (3) and guttiferone E (7) are promising inhibitors of NO production and 15-LOX activity. Further studies should be considered in order to elucidate the mechanism by which these compounds exert their inhibitory activities. (C) 2015 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available