Journal
JOURNAL OF RADIATION RESEARCH
Volume 52, Issue 6, Pages 685-693Publisher
OXFORD UNIV PRESS
DOI: 10.1269/jrr.11015
Keywords
HZE; Secondary particles; 53BP1 foci; Keratinocytes
Funding
- National Aeronautics and Space Administration Department of Energy with the Brookhaven National Laboratory [NNJ08HB63I, DE-AC02-98CH10886]
Ask authors/readers for more resources
High-energy protons, and high mass and energy ions, along with the secondary particles they produce, are the main contributors to the radiation hazard during space explorations. Skin, particularly the epidermis, consisting mainly of keratinocytes with potential for proliferation and malignant transformation, absorbs the majority of the radiation dose. Therefore, we used normal human keratinocytes to investigate and quantify the DNA damage caused by secondary radiation. Its manifestation depends on the presence of retinol in the serum-free media, and is regulated by phosphatidylinositol 3-kinases. We simulated the generation of secondary radiation after the impact of protons and iron ions on an aluminum shield. We also measured the intensity and the type of the resulting secondary particles at two sample locations; our findings agreed well with our predictions. We showed that secondary particles inflict DNA damage to different extents, depending on the type of primary radiation. Low-energy protons produce fewer secondary particles and cause less DNA damage than do high-energy protons. However, both generate fewer secondary particles and inflict less DNA damage than do high mass and energy ions. The majority of cells repaired the initial damage, as denoted by the presence of 53BP1 foci, within the first 24 hours after exposure, but some cells maintained the 53BP1 foci longer.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available