Journal
JOURNAL OF PROTEOMICS
Volume 100, Issue -, Pages 37-43Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jprot.2013.10.023
Keywords
Affinity-purification; Protein-protein interaction; Probabilistic scoring
Categories
Funding
- National University of Singapore
- NIH [R01-GM-094231]
Ask authors/readers for more resources
Significance Analysis of INTeractome (SAINT) is a statistical method for probabilistically scoring protein-protein interaction data from affinity purification-mass spectrometry (AP-MS) experiments. The utility of the software has been demonstrated in many protein-protein interaction mapping studies, yet the extensive testing also revealed some practical drawbacks. In this paper, we present a new implementation, SAINTexpress, with simpler statistical model and quicker scoring algorithm, leading to significant improvements in computational speed and sensitivity of scoring. SAINTexpress also incorporates external interaction data to compute supplemental topology-based scores to improve the likelihood of identifying co-purifying protein complexes in a probabilistically objective manner. Overall, these changes are expected to improve the performance and user experience of SAINT across various types of high quality datasets. Biological significance We present SAINTexpress, an upgraded implementation of Significance Analysis of INTeractome (SAINT) for filtering high confidence interaction data from affinity purification-mass spectrometry (AP-MS) experiments. SAINTexpress features faster computation and incorporation of external data sources into the scoring, improving the performance and user experience of SAINT across various types of datasets. This article is part of a Special Issue entitled: Can Proteomics Fill the Gap Between Genomics and Phenotypes? (C) 2013 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available