Journal
JOURNAL OF PROTEOMICS
Volume 79, Issue -, Pages 123-132Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jprot.2012.12.010
Keywords
Galectin-3; Minute virus of mice; Parvovirus; Proteomics; Viral cell entry; Virus
Categories
Funding
- Canadian Institutes of Health Research
- Natural Sciences and Engineering Research Council of Canada
- Michael Smith Foundation for Health Research
- Canada Foundation for Innovation
- BC Knowledge Development Fund
- BC Proteomics Network
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Cellular factors associated with the parvovirus minute virus of mice (MVM) during infection are thought to play important roles in the MVM life cycle but only a few of these have been identified. Here we used a proteomic-based approach in order to identify host-binding partners of MVM. Using purified MVM as bait for immunoprecipitation assays, a total of 150 proteins were identified in MVM immunoprecipitates by quantitative liquid chromatography-tandem mass spectrometry. Galectin-3 was one of six proteins showing a statistically significant enrichment across replicates. Small interfering RNA depletion studies revealed an important role for galectin-3 in MVM endocytosis and infectivity in LA9 mouse fibroblast cells. Galectin-3-depleted cells were less susceptible to MVM infection than control cells and showed a significant reduction of MVM cellular uptake, but not of MVM binding to the cell surface. Our results indicate an important role for galectin-3 in the cellular uptake of MVM. We propose that galectin-3 facilitates the access of MVM to its receptor(s) at the plasma membrane and in this way promotes MVM endocytosis. (C) 2012 Elsevier B.V. All rights reserved.
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