Journal
JOURNAL OF PROTEOMICS
Volume 75, Issue 3, Pages 938-948Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jprot.2011.10.010
Keywords
Dendritic cell; iTRAQ; Parasitic helminth; Plasma membrane proteomics
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Funding
- UK Biotechnology and Biological Sciences Research Council [BBS/B/08531, BB/C516328]
- Wellcome Trust [071762, 072255]
- European Union [INCO-CT-2006-032405]
- COLFUTURO
- Departamento Administrativo de Ciencia, Tecnologia e Innovacion de la Republica de Colombia (COLCIENCIAS)
- BBSRC
- MRC [G0701437] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/B/08531] Funding Source: researchfish
- Medical Research Council [G0701437] Funding Source: researchfish
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Dendritic cells (DCs) play a pivotal role in polarising Th lymphocyte subsets but it is unclear what molecular events occur when DCs generate Th2-type responses. Here, we analysed plasma membrane-enriched fractions from immature, pro-Th1 and pro-Th2 DCs and used a combination of iTRAQ labelling and LC MS/MS to quantify changes in the proteomes. Analysis was performed on triplicate biological samples and changes verified by flow cytometry. MHC class II molecules and CD29 were up-regulated in pro-Th1 DCs whilst CD18 and CD44 were up-regulated in pro-Th2 DCs. One of the most down-regulated molecules in pro-Thl DCs was YM-1 whilst the greatest decrease in pro-Th2 DCs was NAP-22. Other molecules up-regulated in pro-Th2 DC compared to pro-Thl DCs included some potentially involved in protein folding during antigen processing (clathrin and Rab-7), whilst other non-membrane proteins such as enzymes/transporters related to cell metabolism (malate dehydrogenase, pyruvate kinase, and ATPase Na+/K+ were also recorded. This suggests that pro-Th2 DCs are more metabolically active while pro-Th1 DCs have a mature 'end state'. Overall, although several molecules were preferentially expressed on pro-Th2 DCs, our proteomics data support the view of a 'limited maturation' of pro-Th2 DCs compared to pro-Th1 DCs. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
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