Journal
JOURNAL OF PROTEOMICS
Volume 73, Issue 7, Pages 1404-1409Publisher
ELSEVIER
DOI: 10.1016/j.jprot.2010.02.008
Keywords
Candida albicans cell wall; LC-MALDI TOF/TOF; Surface proteins digestion in vivo; Yeast surfome
Categories
Funding
- Genoma Espana
- Comision Interministerial de Ciencia y Tecnologia (CYCIT, Spain) [BIO 01989-2006, BIO 07654-2009]
- Comunidad Autonoma de Madrid
- REIPI
- Spanish Network for the Research in Infectious Diseases [RD06/0008/1027]
- Instituto de Salud Carlos III C Nombela
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We have set up a fast and easy methodology to identify cell-surface proteins in live yeasts A non-gel proteomic approach was based on a short period of trypsin treatment followed by peptide separation and identification using nano-LC followed by off-line MS/MS Candida albicans was used as a model organism and proteins involved in cell wall organization, cell rescue, defense, virulence, transport, protein fate and metabolism were identified This strategy is a powerful tool to study host-pathogen interactions and to look for potential vaccine candidates and drug targets (C) 2010 Elsevier B V All rights reserved
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