Journal
JOURNAL OF PROTEOMICS
Volume 73, Issue 6, Pages 1241-1253Publisher
ELSEVIER
DOI: 10.1016/j.jprot.2010.02.020
Keywords
2D-E; Mammary gland; Bisphenol A; Biomarkers; Carcinogenesis; Proteomics
Categories
Funding
- NCI NIH HHS [U01 ES/CA 012771] Funding Source: Medline
Ask authors/readers for more resources
Bisphenol A (BPA) is a ubiquitous environmental contaminant with established endocrine disruptor properties. The objective of our study was to determine the effects of prenatal exposure to BPA on the rat mammary gland proteome in postnatal rats as a first step toward the investigation of translational biomarkers of susceptibility in the human population. Pregnant rats were treated orally with 0, 25 or 250 mu g BPA/kg body weight from days 10 to 21 post-conception. Female offspring were euthanized at 21 and 50 days, and mammary glands were collected. Proteomic analysis was conducted using 2-DE, followed by a combination of MALDI-TOF-TOF and LC-MS/MS, which led to the identification of 21 differentially abundant proteins including vimentin, SPARC and 14-3-3. Western blot analysis of key downstream signaling proteins demonstrated increased phospho-AKT, c-Raf, phospho-ERKs-1 and 2, but decreased TGF-beta in mammary glands of 50 day old rats exposed prenatally to BPA. Our studies indicate for the first time that key proteins involved in signaling pathways such as cellular proliferation are regulated at the protein level by BPA. This data is expected to aid in the understanding of how BPA may be influencing the susceptibility of the mammary gland to cancer transformation. (C) 2010 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available