Journal
JOURNAL OF PROTEOMICS
Volume 73, Issue 8, Pages 1433-1440Publisher
ELSEVIER
DOI: 10.1016/j.jprot.2010.01.009
Keywords
2D PAGE; Genetically modified organisms; Soybean; Biomarkers
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Considering the importance of bidimensional electrophoresis and image analysis in comparative proteomics, the parameters that influence the analysis of protein expression of transgenic and non-transgenic soybean seeds were evaluated. The loaded mass of the proteins (150-500 mu g), the pH separation range (3-10 or 4-7), and manual/automatic image editing were evaluated. Additionally, after optimizing the conditions, histograms and matchings were obtained in order to accurately analyze the variations (90%) in protein expression. From this, 10 proteins displayed significant differences in expression, and eight of them were characterized and identified by mass spectrometry. (C) 2010 Elsevier B.V. All rights reserved.
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