Journal
JOURNAL OF PROTEOME RESEARCH
Volume 13, Issue 9, Pages 4028-4039Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr500353x
Keywords
Probiotic; Lactobacillus plantarum; acid stress; gel-free shotgun nanoLC-MS/MS proteomics
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Funding
- Stellenbosch University Subcommittee B Postdoctoral fellowship
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Acid tolerance is considered an important characteristic of probiotic bacteria. Lactobacillus plantarum 423 tolerates acidic pH and is the ideal candidate in which to study molecular mechanisms that add-tolerant lactic acid bacteria employ to survive such conditions. In this study we recorded changes in the protein profile of L. plantarum 423 when exposed to pH 2.5 by using a gel-free nanoLC MS/MS proteomics approach. In total, 97 proteins were detected as more abundant, and 12 proteins were detected solely when strain 423 was exposed to pH 2.5. General stress response proteins, the utilization of a variety of carbohydrate sources in a glucose rich environment, altered pyruvate metabolism, increased lysine biosynthesis, and a significant oxidative stress response was observed in acid-stressed cells. The accumulation of basic compounds also seemed to play an integral role in the response to add stress. We observed a marked decrease in proteins involved in cell wall and phospholipid biosynthesis, transcription, translation, and cell division. The most abundant protein detected was an uncharacterized protein, JDM12142. Functional analysis revealed that this protein plays a role in survival during add stress. Our results contribute to the growing body of knowledge on the molecular mechanisms employed by lactobacilli, in particular L. plan tarum, to ensure survival in acidic conditions.
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