Journal
JOURNAL OF PROTEOME RESEARCH
Volume 13, Issue 7, Pages 3386-3396Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr5002548
Keywords
glandular trichomes; iTRAQ; proteomics; Nicotiana tabacum; trichome purification; MudPIT; MALDI-MS/MS
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Funding
- European Commission [222716]
- Belgian National Fund for Scientific Research
- Interuniversity Poles of Attraction Program (Belgian State, Scientific, Technical and Cultural Services)
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Leaf glandular trichomes (epidermal hairs) actively synthesize secondary metabolites, many of which are the frontline of plant defense. In Nicotiana tabacum, tall and short glandular trichomes have been identified. While the former have been extensively studied and match the classic picture of trichome function, the short trichomes have remained relatively uncharacterized. We have set up a procedure based on centrifugation on Percoll density gradients to obtain separate tall and short trichome fractions purified to >85%. We then investigated the proteome of both trichome types combining 2D-LC fractionation of tryptic peptides and quantification of a set of 461 protein groups using isobaric tags for relative and absolute quantitation. Almost the entire pathway leading to the synthesis of diterpenes was identified in the tall trichomes. Indications for their key roles in the synthesis of cuticular compounds were also found. Concerning the short glandular trichomes, ribosomal proteins and enzymes such phosphoenolpyruvate carboxykinase and polyphenol oxidase were more abundant than in the tall glandular trichomes. These results are discussed in the frame of several hypotheses regarding the respective roles of short and long glandular trichomes.
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