Journal
JOURNAL OF PROTEOME RESEARCH
Volume 13, Issue 12, Pages 6087-6095Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr500992r
Keywords
lysine succinylation; succinylome; lysine succinylation motif; Toxoplasma gondii; tachyzoite
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Funding
- Natural Science Foundation of Zhejiang Province [LY12H19004, LQ14H190003]
- Commonweal Technology Application Project Program of Zhejiang Province [2014C33161]
- National Natural Sciences Foundation [31271362]
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Lysine succinylation is a new posttranslational modification identified in histone proteins of Toxoplasma gondii, an obligate intracellular parasite of the phylum Apicomplexa. However, very little is known about their scope and cellular distribution. Here, using LC-MS/MS to identify parasite peptides enriched by immunopurification with succinyl lysine antibody, we produced the first lysine succinylome in this parasite. Overall, a total of 425 lysine succinylation sites that occurred on 147 succinylated proteins were identified in extracellular Toxoplasma tachyzoites, which is a proliferative stage that results in acute toxoplasmosis. With the bioinformatics analysis, it is shown that these succinylated proteins are evolutionarily conserved and involved in a wide variety of cellular functions such as metabolism and epigenetic gene regulation and exhibit diverse subcellular localizations. Moreover, we defined five types of definitively conserved succinylation site motifs, and the results imply that lysine residue of a polypeptide with lysine on the +3 position and without lysine at the -1 to +2 position is a preferred substrate of lysine succinyltransferase. In conclusion, our findings suggest that lysine succinylation in Toxoplasma involves a diverse array of cellular functions, although the succinylation occurs at a low level.
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