Journal
JOURNAL OF PROTEOME RESEARCH
Volume 13, Issue 12, Pages 5376-5390Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr500173p
Keywords
transmissible gastroenteritis virus (TGEV); isobaric tags for relative and absolute quantitation (iTRAQ); proteomics; interferon signaling
Categories
Funding
- National Natural Sciences Foundation of China [31225027, 31402181, 31121004]
- Fundamental Research Funds for the Central Universities [2013PY043]
Ask authors/readers for more resources
Transmissible gastroenteritis virus (TGEV), a porcine enteropathogenic coronavirus, causes lethal watery diarrhea and severe dehydration in piglets. In this study, liquid chromatographytandem mass spectrometry coupled to isobaric tags for relative and absolute quantification labeling was used to quantitatively identify differentially expressed cellular proteins after TGEV infection in PK-15 cells. In total, 162 differentially expressed cellular proteins were identified, including 60 upregulated proteins and 102 downregulated proteins. These differentially expressed proteins were involved in the cell cycle, cellular growth and proliferation, the innate immune response, etc. Interestingly, many upregulated proteins were associated with interferon signaling, especially signal transducer and activator of transcription 1 (STAT1) and interferon-stimulated genes (ISGs). Immunoblotting and real-time quantitative reverse transcription polymerase chain reaction demonstrated that TGEV infection induces STAT1 phosphorylation and nuclear translocation, as well as ISG expression. This study for the first time reveals that TGEV induces interferon signaling from the point of proteomic analysis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available