Journal
JOURNAL OF PROTEOME RESEARCH
Volume 12, Issue 10, Pages 4414-4423Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr400442y
Keywords
multispecific proteases; nonspecific proteases; site-specific glycosylation; glycoproteomics; biopharmaceutical glycoproteins; glycan isomers
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Funding
- National Research Foundation of Korea
- Converging Research Center Program via the Ministry of Education, Science and Technology [2012K001505]
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Despite recent advances, site-specific profiling of protein glycosylation remains a significant analytical challenge for conventional proteomic methodology. To alleviate the issue, we propose glyco-analytical multispecific proteolysis (Glyco-AMP) as a strategy for glycoproteomic characterization. Glyco-AMP consists of rapid, in-solution digestion of an analyte glycoprotein (or glycoprotein mixture) by a multispecific protease (or protease cocktail). Resulting glycopeptides are chromatographically separated by isomer-specific porous graphitized carbon nano-LC,. quantified by high-resolution MS, and structurally elucidated by MS/MS. To demonstrate the consistency and customizability of Glyco-AMP methodology, the glyco-analytical performances of multispecific proteases subtilisin, pronase, and proteinase K were characterized in terms of quantitative accuracy, sensitivity, and digestion kinetics. Glyco-AMP was shown be effective on glycoprotein mixtures as well as glycoproteins with multiple glycosylation sites, providing detailed, quantitative, site- and structure-specific information about protein glycosylation.
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