Journal
JOURNAL OF PROTEOME RESEARCH
Volume 10, Issue 9, Pages 4018-4032Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr200224x
Keywords
kinase profiling; signal transduction; host-pathogen interaction
Categories
Funding
- CEU [LSHG-CT-2006-037469, PITN-GA-2008-215524]
- TI Pharma [T4-213]
- DFG [SFB/TR34, FOR585]
Ask authors/readers for more resources
Staphylococcus aureus is a dangerous opportunistic human pathogen that causes serious invasive diseases when it reaches the bloodstream. Recent studies have shown that S. aureus is highly resistant to killing by professional phagocytes and that such cells even provide a favorable environment for intracellular survival of S. aureus. Importantly, the reciprocal interactions between phagocytes and S. aureus have remained largely elusive. Here we have employed kinase profiling to define the nature and time resolution of the human THP-1 macrophage response toward S. aureus and proteomics to identify the response of S. aureus toward macrophages. The results of these studies reveal major macrophage signaling pathways triggered by S. aureus and proteomic signatures of the responses of S. aureus to macrophages. We also identify human proteins bound to S. aureus that have potential roles in bacterial killing and internalization. Most noticeably, our observations challenge the classical concept that macrophage responses are mainly mediated through Toll-like receptor 2 and NF-kappa B signaling and highlight the important role of the stress-activated MAP kinase signaling in orchestrating the host defense.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available