Journal
JOURNAL OF PROTEOME RESEARCH
Volume 10, Issue 7, Pages 2992-3002Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr101177g
Keywords
Salmonella enterica serovar Typhimurium; PhoP/PhoQ system; SILAC; quantitative proteomics; low Mg2+ concentrations; cationic antimicrobial peptides
Categories
Funding
- National Basic Research Program of China [2006CB504300, 2007CB914200]
- National Science Foundation of China [30921001]
- PCSIRT [IRT0745]
- 111 Project of China [B06018]
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The PhoP/PhoQ two-component system plays a central regulatory role in the pathogenesis of Salmonella enterica serovar Typhimurium (S. Typhimurium), and it can be activated by low Mg2+ concentrations and sublethal concentrations of cationic antimicrobial peptides (CAMP). Therefore, these two PhoP/PhoQ activation signals are considered as in vivo environmental cues sensed by S. Typhimurium for adaptation and survival. In this work, we conducted a SILAC (stable isotope labeling by amino acids in cell culture)-based quantitative proteomic study to survey the proteomic changes of S. Typhimurium in response to low Mg2+ concentrations or CAMP. We discovered that CAMP activated a portion of the PhoP/PhoQ regulatory network, whereas low Mg2+ concentrations upregulated nearly all known members of this network, a number of previously unknown proteins, and some proteins regulated by IHF and RpoS. Systematic analysis following metabolic pathways revealed that low Mg2+ concentrations selectively influenced, proteins of certain metabolic functions while CAMP did not. Our study indicates that the low Mg2+ concentration condition may lead S. Typhimurium into a growth control lifestyle,which provides new perspectives about Salmonella's adaptation to the host environment.
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