Journal
JOURNAL OF PROTEOME RESEARCH
Volume 9, Issue 11, Pages 5739-5747Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr100525a
Keywords
decidualization; human endometrial stromal cells; src substrate cortactin 8
Categories
Funding
- National Health of Medical Research Council of Australia [41117, 611804, 494808]
- Victorian Government
- CONRAD CICCR, U.S.A
- Ovarian Cancer Research Foundation
Ask authors/readers for more resources
Decidualization is a tissue remodelling process within the uterus in preparation for embryo implantation and pregnancy. In this study we isolated primary human endometrial stromal cells and stimulated decidualization with cAMP. We then used 2D- differential in-gel electrophoresis (DIGE) to identify proteins induced by decidualization. Eighty-eight out of 2714 spots were differentially regulated, 18 of which were assigned clear identities by mass spectrometry. Many of these are proteins known to be associated with cell structure and cytoskeletal remodelling. We validated five of these proteins by Western blot and immunohistochemistry on human endometrial tissue. The validated proteins are caldesmon 1, src substrate contactin 8, tropomyosin alpha-4 chain, protein disulfide isomerase 1A, and LIM and SH3 domain protein. With the exception of caldesmon 1, none of the identified proteins have previously been associated with decidualization. This study provides insight into our understanding of decidualization, which is important for successful embryo implantation and establishment of pregnancy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available