Journal
JOURNAL OF PROTEOME RESEARCH
Volume 9, Issue 12, Pages 6523-6534Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr100727m
Keywords
Dengue; metabolomics; metabolic profiling; EA hy926; H-1 NMR; MS
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Funding
- National University of Singapore, Ministry of Education [R-143-000-293-112, R-143-000 382-112, R-143 000-416-232]
- A STAR [SERC PSF 052 101 0044]
- Singapore Bioimaging Consortium [SBIC 009/2005]
- National Research Foundation
- Economic Development Board (SPORE)
- Ministry of Defense [R-143 000 416-646]
- Environment and Water Industry Development Council [0601 IRIS- 093-08]
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This paper describes a multiplatform analytical approach combining proton nuclear magnetic resonance (H-1 NMR) Spectroscopy and mass spectrometry (MS) together with pattern recognition tools in a metabolomic study used to investigate the effects of dengue virus infection The four serotypes of dengue, DEN-1, DEN-2, DEN-3, and DEN-4, were inoculated into the EA hy926 cell line, which was then incubated for various time intervals Principal component analysis (PCA) of the H-1 NMR and MS data revealed metabolic profile patterns or fingerprint patterns that can be attributed to specific virus serotypes Distinct effects of infection by each serotype were demonstrated, and these differences were attributed to changes in levels of metabolites (including amino acids, dicarboxylic acids, fatty acids and organic acids related to the tricarboxylic acid (TCA) cycle) The study demonstrated application of metabolomics to improve understanding of the effect of dengue infection on endothelial cells' metabolome
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