Journal
JOURNAL OF PROTEOME RESEARCH
Volume 9, Issue 6, Pages 2825-2838Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr900866e
Keywords
Mitochondria; Alternative oxidase (AOX); Proteomics; Metabolism
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Funding
- Belgian Fonds de la Recherche Scientifique FNRS (F.R.S.-FNRS) [1.C057.09, F.4735.06, FRFC 2.4582.05]
- Action de Recherche Concertee [ARC07/12-04]
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In the present work, we have isolated by RNA interference and characterized at the functional and the proteomic levels a Chlamydomonas reinhardtii strain devoid of the mitochondrial alternative oxidase 1 (AOX1). The AOX1-deficient strain displays a remarkable doubling of the cell volume and biomass without alteration of the generation time or change in total respiratory rate, with a significantly higher ROS production. To identify the molecular adaptation underlying these observations, we have carried out a comparative study of both the mitochondrial and the cellular soluble proteomes. Our results indicate a strong up-regulation of the ROS scavenging systems and important quantitative modifications of proteins involved in the primary metabolism, namely an increase of enzymes involved in anabolic pathways and a concomitant general down-regulation of enzymes of the main catabolic pathways.
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