Journal
JOURNAL OF PROTEOME RESEARCH
Volume 9, Issue 1, Pages 40-49Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr9001367
Keywords
translationally controlled tumor protein (TCTP); proteomics; metastasis; mass spectrometry; MALDI-TOF/TOF-MS; 2D gel electrophoresis
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Funding
- National High Technology Research and Development Program of China [2006AA02A311]
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Translationally controlled tumor protein (TCTP) plays a major role in a broad array of biological processes. However, the TCTP-related biological process and interactive proteins still remain poorly characterized. In the present study, we found that knockdown of TCTP inhibited proliferation, migration, and invasion activities of LoVo cells in vitro and in vivo. The whole-cell proteomes were compared by 2D gel electrophoresis before and after knockdown of TCTP. Alterations in 27 proteins were detected and their identities were revealed by mass spectrometry analysis. Components of Ubiquitin-Proteasome System, proteins involved in the cytoskeleton biosynthesis and tumor metastasis were found to be changed upon TCTP removal. These results imply that TCTP might play at least a partial role in colon adenocarcinoma progression.
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