Journal
JOURNAL OF PROTEOME RESEARCH
Volume 9, Issue 12, Pages 6256-6264Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr1004828
Keywords
2D-DiGE; human endometrial secretions; infertility; antithrombin III; alpha-2 macroglobulin; uterine lavage
Categories
Funding
- NHMRC (Australia) [494802]
- Victorian Government
- NHMRC [388901]
- Ovarian Cancer Research Foundation
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Endometrial secretions in the uterine cavity contain mediators important for endometrial receptivity and embryo implantation Unbiased analysis of uterine fluid from a receptive versus nonreceptive time of the menstrual cycle and in fertile and infertile women will provide new insights into uterine receptivity We hypothesized that proteomic analysis of human uterine lavages would identify proteins important for the establishment of pregnancy in humans Lavages collected from fertile (n = 7) and infertile (n = ) women during the midsecretory (MS) phase, and from fertile women during the midproliferative (MP) (n = 7) phase, were assessed using 2D-differential in gel electrophoresis (2D-DiGE) over a p/4-7 range Statistical analysis revealed 7 spots that were significantly decreased in the MP compared to the MS phase, while 18 spots showed differential expression between fertile and infertile women A number of proteins were identified by mass spectrometry, including antithrombin Ill and alpha-2-macroglobulin, whose production was confirmed in endometrial epithelium Their staining pattern suggests roles during embryo implantation Assessment of the human endometrial secretome has identified differences in the protein content of uterine fluid with respect to receptivity and fertility
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