Journal
JOURNAL OF PROTEOME RESEARCH
Volume 9, Issue 12, Pages 6722-6728Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr100868d
Keywords
ribosomal protein; MALDI-TOF MS; bacterial identification; S10 spc alpha operon
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Funding
- Ministry of Education Culture, Sports Science and Technology
- Japan Society for the Promotion of Science
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We have proposed a rapid phylogenetic classification at the strain level by MALDI TOF MS using ribosomal protein matching profiling In this study the S10-spc-alpha operon, encoding half of the ribosomal subunit proteins and highly conserved in eubacterial genomes was selected for construction of the ribosomal protein database as biomarkers for bacterial identification by MALDI-TOF MS analysis to establish a more reliable phylogenetic classification Our method revealed that the 14 reliable and reproducible ribosomal subunit proteins with less than m/z 15 000 except for L14, coded in the S10-spc-alpha operon were significantly useful biomarkers for bacterial classification at species and strain levels by MALDI TOF MS analysis of genus Pseudomonas strains The obtained phylogenetic tree was consisted with that based on genetic sequence (gyrB) Since S10-spc-alpha operons of genus Pseudomonas strains were sequenced using specific primers designed based on nucleotide sequences of genome sequenced strains, the ribosomal subunit proteins encoded in S10-spc-alpha operon were suitable biomarkers for construction and correction of the database MALDI TOF MS analysis using these 14 selected ribosomal proteins is a rapid efficient, and versatile bacterial identification method with the validation procedure for the obtained results
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