Journal
JOURNAL OF PROTEOME RESEARCH
Volume 9, Issue 2, Pages 876-884Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr900780s
Keywords
phosphoproteomics; human plasma; biomarkers; phosphopeptide purification; combined search engines
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Funding
- Spanish Ministerio de Ciencia e Innovacion [BIO2008-03369]
- Genoma Spain
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Major plasma protein families play different roles in blood physiology and hemostasis and in immunodefense. Other proteins in plasma can be involved in signaling as chemical messengers or constitute biological markers of the status of distant tissues. In this respect, the plasma phosphoproteome holds potentially relevant information on the mechanisms modulating these processes through the regulation of protein activity. In this work we describe for the first time a collection of phosphopeptides identified in human plasma using immunoaffinity separation of the seven major serum protein families from other plasma proteins, SCX fractionation, and TiO2 purification prior to LC-MS/MS analysis. One-hundred and twenty-seven phosphosites in 138 phosphopeptides mapping 70 phosphoproteins were identified with FDR < 1%. A high-confidence collection of phosphosites was obtained using a combined search with the OMSSA, SEQUEST, and Phenyx search engines.
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