Participation of NMDA receptors in the lateral hypothalamus in gastric erosion induced by cold-water restraint

The present study investigated whether neurons in the lateral hypothalamus (LH) play a role in the occurrence of gastric ulcerations induced by cold-water restraint. The first experiment indicated that bilateral N-methyl-D-aspartate (NMDA) lesions of the LH (20 mu g/1 mu l per side) reduced the amount of gastric ulceration induced by cold-water restraint. In the second experiment, the NMDA antagonist DL-2-amino-5-phosphonovaleric acid (APV; 2.5 mu g/0.5 mu l per side) or its vehicle was microinjected bilaterally into the LH prior to the cold-water restraint procedure. APV did not induce gastric ulcerations but reduced the amount of ulceration induced by cold-water restraint. These results indicate that NMDA receptors in the LH play an important role in the occurrence of gastric ulceration induced by cold-water restraint. The participation of the LH and possible neuronal circuitry involved in stress-induced ulceration are discussed. (C) 2014 Elsevier Inc. All rights reserved.