Journal
JOURNAL OF PROTEOME RESEARCH
Volume 8, Issue 3, Pages 1404-1414Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr8010155
Keywords
Cerebrospinal fluid; differentially abundant peptides; multiple sclerosis; proteomics; innate immunity; clinically isolated syndrome
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Funding
- Netherlands Multiple Sclerosis Research Foundation
- Netherlands Organization for Scientific research
- Netherlands Proteomics Centre
- Top Institute Pharma [D4-102-1]
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We introduce a matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FT-ICR) method for quantitative peptide profiling, using peak height as a measure for abundance. Relative standard deviations in peak height of peptides spiked over 3 orders of magnitude in concentration were below 10% and allowed for accurate comparisons between multiple sclerosis and controls. Application on a set of 163 cerebrospinal fluid (CSF) samples showed significantly differential abundant peptides, which were subsequently identified into proteins (e.g., chromogranin A, clusterin, and complement C3).
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