Journal
JOURNAL OF PROTEOME RESEARCH
Volume 8, Issue 7, Pages 3712-3726Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr900320a
Keywords
phosphoinositides; proteomics; interactome; microaffinity purification
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Funding
- Australian Research Council
- Discovery Project [DP0770668]
- CSIRO
- VESKI (Victorian Endowment for Science, Knowledge and Innovation)
- University of Pennsylvania, Philadelphia, PA
- Australian Research Council [DP0770668] Funding Source: Australian Research Council
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Immobilizing chemically synthesized analogues of PI(3,4,5)P3 onto Affi-10 beads and incorporating them into liposomes allowed their use as affinity absorbents in the comprehensive analysis of the phosphoinositide interactome using cytosolic cell extracts of the LIM1215 colon cancer cell line. This led to the identification of 282 proteins that either interact with PI(3,4,5)P3 or are indirectly captured as part of a complex containing a PI(3,4,5)P3 binding partner. Identification of the proteins was achieved using affinity/LC-MS/MS experiments.
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