Journal
JOURNAL OF PROTEOME RESEARCH
Volume 8, Issue 7, Pages 3617-3624Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr800816w
Keywords
lectin; Maackia amurensis hemagglutinin; IgA nephropathy; phage display; cluster analysis
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Funding
- Ministry of Education, Science, Sports, and Culture of Japan [1.1557180, 11672162, 12307054]
- Research Association for Biotechnology, by the Program for Promotion of Basic Research Activities for Innovative Biosciences
- Program for Promotion of Fundamental Studies in Health Sciences
- Pharmaceutical and Medical Device Agency
- Grants-in-Aid for Scientific Research [12307054, 11672162] Funding Source: KAKEN
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Ten genetically modified Maackia amurensis hemagglutinin (MAH) clones at the carbohydrate-recognition loop were found to bind glycophorin A and a mucin mimetic with NeuAc(12-3GaI beta 1 -3GaINAc alpha( (monosialyl-T antigen) in different relative intensity. Binding profiles of these lectins to human serum IgA1 from healthy individuals and from IgA nephropathy patients were subjected to the cluster analysis. Two large groups, one with only healthy individuals and another with all IgA nephropathy patients, were generated. The results strongly suggest that the library of genetically modified MAH is a useful tool for serum diagnosis of IgA nephropathy.
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