Journal
JOURNAL OF PROTEOME RESEARCH
Volume 8, Issue 11, Pages 5031-5040Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr900452s
Keywords
Carbohydrates; Carbohydrate Microarray; Glycan; Glycan arrays; Glycomics; Oligosaccaharides; Microwave assisted reaction; Polysaccharides; Postgenomics
Categories
Funding
- National Center for Research Resources Services (NCRR) [1R43 RR023763-01, 2R44 RR023763-02]
- National Cancer Institute (NCI) [U01CA128416]
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We reported here a novel, ready-to-use bioarray platform and methodology for construction of sensitive carbohydrate cluster microarrays This technology utilizes a three-dimensional (3-D) poly(amidoamine) starburst dendrimer monolayer assembled on glass surface, which is functionalized with terminal aminooxy and hydrazide groups for site-specific coupling of carbohydrates A wide range of saccharides, including monosaccharides, oligosaccharides and polysaccharides of diverse structures, are applicable for the 3-D bioarray platform without prior chemical derivatization The process of carbohydrate coupling is effectively accelerated by microwave radiation energy The carbohydrate concentration required for microarray fabrication is substantially reduced using this technology. Importantly, this bioarray platform presents sugar chains in defined orientation and cluster configurations. It is, thus, uniquely useful for exploration of the structural and conformational diversities of glyco-epitope and their functional properties.
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